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Single Cell Rna Qc

技能 活跃

Performs quality control on single-cell RNA-seq data (.h5ad or .h5 files) using scverse best practices with MAD-based filtering and comprehensive visualizations. Use when users request QC analysis, filtering low-quality cells, assessing data quality, or following scverse/scanpy best practices for single-cell analysis.

目的

To automate the quality control of single-cell RNA-seq data, ensuring data integrity and readiness for downstream analysis by applying scverse best practices.

功能

  • Automated QC pipeline for single-cell RNA-seq
  • Supports .h5ad and .h5 file formats
  • MAD-based filtering and comprehensive visualizations
  • Modular functions for custom workflows
  • Follows scverse/scanpy best practices

使用场景

  • Performing QC analysis on single-cell RNA-seq data
  • Filtering low-quality cells based on various metrics
  • Assessing overall data quality through visualizations
  • Following scverse/scanpy best practices for single-cell analysis

非目标

  • Performing downstream analysis like normalization or clustering
  • Ambient RNA correction or doublet detection (these are mentioned as next steps)
  • Batch correction or cell cycle scoring

Maintenance

  • warning:Dependency ManagementThe script relies on common Python libraries (anndata, scanpy, etc.) but there are no explicit dependency pinning or vulnerability checks mentioned.

Trust

  • warning:Issues Attention29 issues opened and 4 closed in the last 90 days indicate a slow response rate to open issues.

Execution

  • warning:Pinned dependenciesWhile Python scripts have shebangs, dependencies like anndata and scanpy are not explicitly pinned in a lockfile, and side-effect headers are missing.

安装

请先添加 Marketplace

/plugin marketplace add anthropics/knowledge-work-plugins
/plugin install bio-research@knowledge-work-plugins

质量评分

75 /100
13 days ago 分析

信任信号

最近提交14 days ago
星标12.1k
许可证Apache-2.0
状态
查看源代码

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